Charlie Hebdo’s office became the prime target of a massacre by Islamist gunmen in 2015.
"We will never lie down. We will never give up," director Laurent "Riss" Sourisseau wrote in an editorial to go with the cartoons in the latest edition.
"The hatred that struck us is still there and, since 2015, it has taken the time to mutate, to change its appearance, to go unnoticed and to quietly continue its ruthless crusade," he said.
Twelve people, including some of France's most celebrated cartoonists, were killed on January 7, 2015, when brothers Said and Cherif Kouachi went on a gun rampage at the paper's offices in Paris.
The perpetrators were killed in the wake of the massacre but 14 alleged accomplices in the attacks, which also targeted a Jewish supermarket, will go on trial in Paris on Wednesday.
The latest Charlie Hebdo cover shows a dozen cartoons first published by the Danish daily Jyllands-Posten in 2005 — and then reprinted by the French weekly in 2006, unleashing a storm of anger across the Muslim world.
In the center of the cover is a cartoon of the prophet drawn by cartoonist Jean Cabut, known as Cabu, who lost his life in the massacre.